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Darwin Assembly: fast, efficient, multi-site bespoke mutagenesis

Engineering proteins for designer functions and biotechnological applications almost invariably requires (or at least benefits from) multiple mutations to non-contiguous residues. Several methods for multiple site-directed mutagenesis exist, but there remains a need for fast and simple methods to ef...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Cozens, Christopher, Pinheiro, Vitor B
Format: Artigo
Language:Inglês
Published: Oxford University Press 2018
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5934624/
https://ncbi.nlm.nih.gov/pubmed/29409059
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky067
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