載入...
A novel PCR strategy for high-efficiency, automated site-directed mutagenesis
We have developed a novel three-primer, one-step PCR-based method for site-directed mutagenesis. This method takes advantage of the fact that template plasmid DNA cannot be efficiently denatured at its reannealing temperature (T(ra)), which is otherwise a troublesome problem in regular PCR. Two flan...
Na minha lista:
| Main Authors: | , , , , |
|---|---|
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Oxford University Press
2005
|
| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1178011/ https://ncbi.nlm.nih.gov/pubmed/16030347 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni115 |
| 標簽: |
添加標簽
沒有標簽, 成為第一個標記此記錄!
|