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Efficient procedure for site-directed mutagenesis mediated by PCR insertion of a novel restriction site
Better understanding of proteins' structure/function relationship and dissecting their functional domains are still challenges yet to be mastered. Site-directed mutagenesis approaches that can alter bases at precise positions on the gene sequence can help to reach this goal. This article descri...
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Landes Bioscience
2010
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3115099/ https://ncbi.nlm.nih.gov/pubmed/21150303 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/psb.5.12.13994 |
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