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Efficient procedure for site-directed mutagenesis mediated by PCR insertion of a novel restriction site

Better understanding of proteins' structure/function relationship and dissecting their functional domains are still challenges yet to be mastered. Site-directed mutagenesis approaches that can alter bases at precise positions on the gene sequence can help to reach this goal. This article descri...

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Bibliografski detalji
Glavni autor: Rouached, Hatem
Format: Artigo
Jezik:Inglês
Izdano: Landes Bioscience 2010
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3115099/
https://ncbi.nlm.nih.gov/pubmed/21150303
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/psb.5.12.13994
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