Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method.
We describe a rapid and efficient megaprimer PCR procedure for site-directed mutagenesis that does not require any intermediate purification of DNA between the two rounds of PCR. This protocol is based on the design of forward and reverse flanking primers with significantly different melting tempera...
Guardat en:
| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1997
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146891/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9241254/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.16.3371 |
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