Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method.

We describe a rapid and efficient megaprimer PCR procedure for site-directed mutagenesis that does not require any intermediate purification of DNA between the two rounds of PCR. This protocol is based on the design of forward and reverse flanking primers with significantly different melting tempera...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Ke, S H, Madison, E L
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1997
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146891/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9241254/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.16.3371
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