Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method.

We describe a rapid and efficient megaprimer PCR procedure for site-directed mutagenesis that does not require any intermediate purification of DNA between the two rounds of PCR. This protocol is based on the design of forward and reverse flanking primers with significantly different melting tempera...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Ke, S H, Madison, E L
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146891/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9241254/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.16.3371
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