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A novel PCR strategy for high-efficiency, automated site-directed mutagenesis
We have developed a novel three-primer, one-step PCR-based method for site-directed mutagenesis. This method takes advantage of the fact that template plasmid DNA cannot be efficiently denatured at its reannealing temperature (T(ra)), which is otherwise a troublesome problem in regular PCR. Two flan...
Gorde:
| Egile Nagusiak: | , , , , |
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| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
Oxford University Press
2005
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1178011/ https://ncbi.nlm.nih.gov/pubmed/16030347 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni115 |
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