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Twin-primer non-enzymatic DNA assembly: an efficient and accurate multi-part DNA assembly method

DNA assembly forms the cornerstone of modern synthetic biology. Despite the numerous available methods, scarless multi-fragment assembly of large plasmids remains challenging. Furthermore, the upcoming wave in molecular biological automation demands a rethinking of how we perform DNA assembly. To st...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Liang, Jing, Liu, Zihe, Low, Xi Z., Ang, Ee L., Zhao, Huimin
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5499748/
https://ncbi.nlm.nih.gov/pubmed/28334760
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx132
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