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Solid-phase cloning for high-throughput assembly of single and multiple DNA parts
We describe solid-phase cloning (SPC) for high-throughput assembly of expression plasmids. Our method allows PCR products to be put directly into a liquid handler for capture and purification using paramagnetic streptavidin beads and conversion into constructs by subsequent cloning reactions. We pre...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4402512/ https://ncbi.nlm.nih.gov/pubmed/25618848 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv036 |
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