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New insights into the QuikChange(TM) process guide the use of Phusion DNA polymerase for site-directed mutagenesis
The QuikChange(TM) site-directed mutagenesis method is popular but imperfect. An improvement by using partially overlapping primers has been reported several times; however, it is incompatible with the proposed mechanism. The QuikChange(TM) method using complementary primers is proposed to linearly...
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| Publicado en: | Nucleic Acids Res |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
2015
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4333370/ https://ncbi.nlm.nih.gov/pubmed/25399421 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku1189 |
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