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Evaluating a Ligation-Mediated PCR and Pyrosequencing Method for the Detection of Clonal Contribution in Polyclonal Retrovirally Transduced Samples

Retroviral gene transfer has proven therapeutic potential in clinical gene therapy trials but may also cause abnormal cell growth via perturbation of gene expression in the locus surrounding the insertion site. By establishing clonal marks, retroviral insertions are also used to describe the regener...

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Detalhes bibliográficos
Main Authors: Brugman, Martijn H., Suerth, Julia D., Rothe, Michael, Suerbaum, Sebastian, Schambach, Axel, Modlich, Ute, Kustikova, Olga, Baum, Christopher
Formato: Artigo
Idioma:Inglês
Publicado em: Mary Ann Liebert, Inc. 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3732125/
https://ncbi.nlm.nih.gov/pubmed/23384086
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/hgtb.2012.175
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