High-Throughput FRET Assay Yields Allosteric SERCA Activators

Using fluorescence resonance energy transfer (FRET), we performed a high-throughput screen (HTS) in a reconstituted membrane system, seeking compounds that reverse inhibition of sarco-/endoplasmic reticulum Ca-ATPase (SERCA) by its endogenous regulator, phospholamban (PLB). Such compounds have long...

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Bibliographic Details
Main Authors: Cornea, Razvan L., Lockamy, Elizabeth L., Gruber, Simon J., Muretta, Joseph M., Jin, Dongzhu, Chen, Jiqiu, Dahl, Russell, Bartfai, Tamas, Zsebo, Krisztina M., Gillispie, Gregory D., Thomas, David D.
Format: Artigo
Language:Inglês
Published: 2012
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3721969/
https://ncbi.nlm.nih.gov/pubmed/22923787
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/1087057112456878
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