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Discovery of enzyme modulators via high-throughput time-resolved FRET in living cells
We have used a “2-color” SERCA (sarco/endoplasmic reticulum calcium ATPase) biosensor and a unique high-throughput fluorescence lifetime plate-reader (FLT-PR) to develop a high-precision live-cell assay designed to screen for small molecules that perturb SERCA structure. A SERCA construct, in which...
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| Autors principals: | , , , , , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2014
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4013825/ https://ncbi.nlm.nih.gov/pubmed/24436077 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/1087057113510740 |
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