Discovery of enzyme modulators via high-throughput time-resolved FRET in living cells

We have used a “2-color” SERCA (sarco/endoplasmic reticulum calcium ATPase) biosensor and a unique high-throughput fluorescence lifetime plate-reader (FLT-PR) to develop a high-precision live-cell assay designed to screen for small molecules that perturb SERCA structure. A SERCA construct, in which...

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Main Authors: Gruber, Simon J., Cornea, Razvan L., Li, Ji, Peterson, Kurt C., Schaaf, Tory M., Gillispie, Gregory D., Dahl, Russell, Zsebo, Krisztina M., Robia, Seth L., Thomas, David D.
Formato: Artigo
Idioma:Inglês
Publicado: 2014
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4013825/
https://ncbi.nlm.nih.gov/pubmed/24436077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/1087057113510740
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