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Red-Shifted FRET Biosensors for High-Throughput Fluorescence Lifetime Screening

We have developed fluorescence resonance energy transfer (FRET) biosensors with red-shifted fluorescent proteins (FP), yielding improved characteristics for time-resolved (lifetime) fluorescence measurements. In comparison to biosensors with green and red FRET pairs (GFP/RFP), FPs that emit at longe...

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Detalles Bibliográficos
Publicado en:Biosensors (Basel)
Main Authors: Schaaf, Tory M., Li, Ang, Grant, Benjamin D., Peterson, Kurt, Yuen, Samantha, Bawaskar, Prachi, Kleinboehl, Evan, Li, Ji, Thomas, David D., Gillispie, Gregory D.
Formato: Artigo
Idioma:Inglês
Publicado: MDPI 2018
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6315989/
https://ncbi.nlm.nih.gov/pubmed/30352972
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/bios8040099
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