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High-throughput spectral and lifetime-based FRET screening in living cells to identify small-molecule effectors of SERCA

A robust high-throughput screening (HTS) strategy has been developed to discover small-molecule effectors targeting the sarco/endoplasmic reticulum calcium ATPase (SERCA), based on a fluorescence microplate reader that records both the nanosecond decay waveform (lifetime mode) and the complete emiss...

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Detalhes bibliográficos
Publicado no:SLAS Discov
Main Authors: Schaaf, Tory M., Peterson, Kurt C., Grant, Benjamin D., Bawaskar, Prachi, Yuen, Samantha, Li, Ji, Muretta, Joseph M., Gillispie, Gregory D., Thomas, David D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5323330/
https://ncbi.nlm.nih.gov/pubmed/27899691
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/1087057116680151
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