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Relative stabilities of IgG1 and IgG4 Fab domains: Influence of the light–heavy interchain disulfide bond architecture

The stability of therapeutic antibodies is a prime pharmaceutical concern. In this work we examined thermal stability differences between human IgG1 and IgG4 Fab domains containing the same variable regions using the thermofluor assay. It was found that the IgG1 Fab domain is up to 11°C more stable...

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Detalhes bibliográficos
Main Authors: Heads, James T, Adams, Ralph, D'Hooghe, Lena E, Page, Matt J T, Humphreys, David P, Popplewell, Andrew G, Lawson, Alastair D, Henry, Alistair J
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3631360/
https://ncbi.nlm.nih.gov/pubmed/22761163
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2118
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