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Azole Affinity of Sterol 14α-Demethylase (CYP51) Enzymes from Candida albicans and Homo sapiens

Candida albicans CYP51 (CaCYP51) (Erg11), full-length Homo sapiens CYP51 (HsCYP51), and truncated Δ60HsCYP51 were expressed in Escherichia coli and purified to homogeneity. CaCYP51 and both HsCYP51 enzymes bound lanosterol (K(s), 14 to 18 μM) and catalyzed the 14α-demethylation of lanosterol using H...

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Bibliographische Detailangaben
Hauptverfasser: Warrilow, Andrew G., Parker, Josie E., Kelly, Diane E., Kelly, Steven L.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3591892/
https://ncbi.nlm.nih.gov/pubmed/23274672
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.02067-12
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