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Direct selection for mutations affecting specific splice sites in a hamster dihydrofolate reductase minigene.

A Chinese hamster cell line containing an extra exon 2 (50 bp) inserted into a single intron of a dihydrofolate reductase (dhfr) minigene was constructed. The extra exon 2 was efficiently spliced into the RNA, resulting in an mRNA that is incapable of coding for the DHFR enzyme. Mutations that decre...

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Hlavní autoři: Chen, I T, Chasin, L A
Médium: Artigo
Jazyk:Inglês
Vydáno: 1993
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC358908/
https://ncbi.nlm.nih.gov/pubmed/8417332
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