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Direct selection for mutations affecting specific splice sites in a hamster dihydrofolate reductase minigene.
A Chinese hamster cell line containing an extra exon 2 (50 bp) inserted into a single intron of a dihydrofolate reductase (dhfr) minigene was constructed. The extra exon 2 was efficiently spliced into the RNA, resulting in an mRNA that is incapable of coding for the DHFR enzyme. Mutations that decre...
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| 發表在: | Mol Cell Biol |
|---|---|
| Main Authors: | , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Taylor & Francis
1993
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC358908/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8417332/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.13.1.289 |
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