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Direct selection for mutations affecting specific splice sites in a hamster dihydrofolate reductase minigene.
A Chinese hamster cell line containing an extra exon 2 (50 bp) inserted into a single intron of a dihydrofolate reductase (dhfr) minigene was constructed. The extra exon 2 was efficiently spliced into the RNA, resulting in an mRNA that is incapable of coding for the DHFR enzyme. Mutations that decre...
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| Main Authors: | , |
|---|---|
| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
1993
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| Ábhair: | |
| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC358908/ https://ncbi.nlm.nih.gov/pubmed/8417332 |
| Clibeanna: |
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