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Direct selection for mutations affecting specific splice sites in a hamster dihydrofolate reductase minigene.

A Chinese hamster cell line containing an extra exon 2 (50 bp) inserted into a single intron of a dihydrofolate reductase (dhfr) minigene was constructed. The extra exon 2 was efficiently spliced into the RNA, resulting in an mRNA that is incapable of coding for the DHFR enzyme. Mutations that decre...

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Udgivet i:Mol Cell Biol
Principais autores: Chen, I T, Chasin, L A
Format: Artigo
Sprog:Inglês
Udgivet: Taylor & Francis 1993
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC358908/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8417332/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.13.1.289-300.1993
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