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Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity.

Mutants of Chinese hamster ovary cells lacking dihydrofolate reductase (tetrahydrofolate dehydrogenase, 7,8-dihydrofolate:NADP+ oxidoreductase; EC 1.5.1.3) activity were isolated after mutagenesis and exposure to high-specific-activity [3H]deoxyuridine as a selective agent. Fully deficient mutants c...

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Detalhes bibliográficos
Main Authors: Urlaub, G, Chasin, L A
Formato: Artigo
Idioma:Inglês
Publicado em: 1980
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC349802/
https://ncbi.nlm.nih.gov/pubmed/6933469
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