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Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM
Here we present a fluctuation-based approach to biosensor Förster resonance energy transfer (FRET) detection that can measure the molecular flow and signaling activity of proteins in live cells. By simultaneous use of the phasor approach to fluorescence lifetime imaging microscopy (FLIM) and cross–p...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
2013
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3538204/ https://ncbi.nlm.nih.gov/pubmed/23248275 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1211882110 |
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