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Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM

Here we present a fluctuation-based approach to biosensor Förster resonance energy transfer (FRET) detection that can measure the molecular flow and signaling activity of proteins in live cells. By simultaneous use of the phasor approach to fluorescence lifetime imaging microscopy (FLIM) and cross–p...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Hinde, Elizabeth, Digman, Michelle A., Hahn, Klaus M., Gratton, Enrico
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3538204/
https://ncbi.nlm.nih.gov/pubmed/23248275
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1211882110
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