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Biosensor FRET detection by the phasor approach to fluorescence lifetime imaging microscopy (FLIM)

We present here the phasor approach to biosensor FRET detection by fluorescence lifetime imaging microscopy (FLIM) and show that this method of data representation is robust towards biosensor design as well as the fluorescence artifacts inherent to the cellular environment. We demonstrate this prope...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Hinde, Elizabeth, Digman, Michelle A., Welch, Christopher, Hahn, Klaus M., Gratton, Enrico
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3523109/
https://ncbi.nlm.nih.gov/pubmed/21858900
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jemt.21054
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