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Biosensor FRET detection by the phasor approach to fluorescence lifetime imaging microscopy (FLIM)

We present here the phasor approach to biosensor FRET detection by fluorescence lifetime imaging microscopy (FLIM) and show that this method of data representation is robust towards biosensor design as well as the fluorescence artifacts inherent to the cellular environment. We demonstrate this prope...

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Detalhes bibliográficos
Main Authors: Hinde, Elizabeth, Digman, Michelle A., Welch, Christopher, Hahn, Klaus M., Gratton, Enrico
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3523109/
https://ncbi.nlm.nih.gov/pubmed/21858900
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jemt.21054
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