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Biosensor FRET detection by the phasor approach to fluorescence lifetime imaging microscopy (FLIM)

We present here the phasor approach to biosensor FRET detection by fluorescence lifetime imaging microscopy (FLIM) and show that this method of data representation is robust towards biosensor design as well as the fluorescence artifacts inherent to the cellular environment. We demonstrate this prope...

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Autori principali: Hinde, Elizabeth, Digman, Michelle A., Welch, Christopher, Hahn, Klaus M., Gratton, Enrico
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2011
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3523109/
https://ncbi.nlm.nih.gov/pubmed/21858900
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jemt.21054
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