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Measurements of absolute concentrations of NADH in cells using the phasor FLIM method

We propose a graphical method using the phasor representation of the fluorescence decay to derive the absolute concentration of NADH in cells. The method requires the measurement of a solution of NADH at a known concentration. The phasor representation of the fluorescence decay accounts for the diff...

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書目詳細資料
發表在:Biomed Opt Express
Main Authors: Ma, Ning, Digman, Michelle A., Malacrida, Leonel, Gratton, Enrico
格式: Artigo
語言:Inglês
出版: Optical Society of America 2016
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4948605/
https://ncbi.nlm.nih.gov/pubmed/27446681
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.7.002441
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