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Measurements of absolute concentrations of NADH in cells using the phasor FLIM method
We propose a graphical method using the phasor representation of the fluorescence decay to derive the absolute concentration of NADH in cells. The method requires the measurement of a solution of NADH at a known concentration. The phasor representation of the fluorescence decay accounts for the diff...
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| Pubblicato in: | Biomed Opt Express |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Optical Society of America
2016
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4948605/ https://ncbi.nlm.nih.gov/pubmed/27446681 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.7.002441 |
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