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Measurements of absolute concentrations of NADH in cells using the phasor FLIM method

We propose a graphical method using the phasor representation of the fluorescence decay to derive the absolute concentration of NADH in cells. The method requires the measurement of a solution of NADH at a known concentration. The phasor representation of the fluorescence decay accounts for the diff...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Biomed Opt Express
Päätekijät: Ma, Ning, Digman, Michelle A., Malacrida, Leonel, Gratton, Enrico
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Optical Society of America 2016
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC4948605/
https://ncbi.nlm.nih.gov/pubmed/27446681
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.7.002441
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