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Manipulating replisome dynamics to enhance lambda Red-mediated multiplex genome engineering
Disrupting the interaction between primase and helicase in Escherichia coli increases Okazaki fragment (OF) length due to less frequent primer synthesis. We exploited this feature to increase the amount of ssDNA at the lagging strand of the replication fork that is available for λ Red-mediated Multi...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3526312/ https://ncbi.nlm.nih.gov/pubmed/22904085 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks751 |
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