Lambda Red Recombineering in Escherichia coli Occurs Through a Fully Single-Stranded Intermediate

The phage lambda-derived Red recombination system is a powerful tool for making targeted genetic changes in Escherichia coli, providing a simple and versatile method for generating insertion, deletion, and point mutations on chromosomal, plasmid, or BAC targets. However, despite the common use of th...

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Bibliographic Details
Main Authors: Mosberg, J. A., Lajoie, M. J., Church, G. M.
Format: Artigo
Language:Inglês
Published: Genetics Society of America 2010
Subjects:
Investigations
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2975298/
https://ncbi.nlm.nih.gov/pubmed/20813883
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.110.120782
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