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Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases
Lambda Red recombineering is a powerful technique for making targeted genetic changes in bacteria. However, many applications are limited by the frequency of recombination. Previous studies have suggested that endogenous nucleases may hinder recombination by degrading the exogenous DNA used for reco...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3434165/ https://ncbi.nlm.nih.gov/pubmed/22957093 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0044638 |
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