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Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli

BACKGROUND: The λ Red recombineering technology has been used extensively in Escherichia coli and Salmonella typhimurium for easy PCR-mediated generation of deletion mutants, but less so in pathogenic species of E. coli such as EHEC and EPEC. Our early experiments with the use of λ Red in EHEC and E...

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Bibliografske podrobnosti
Main Authors: Murphy, Kenan C, Campellone, Kenneth G
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2003
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC317293/
https://ncbi.nlm.nih.gov/pubmed/14672541
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-4-11
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