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Isolation and Quantitation of Topoisomerase Complexes Accumulated on Escherichia coli Chromosomal DNA
DNA topoisomerases are important targets in anticancer and antibacterial therapy because drugs can initiate cell death by stabilizing the transient covalent topoisomerase-DNA complex. In this study, we employed a method that uses CsCl density gradient centrifugation to separate unbound from DNA-boun...
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| Auteurs principaux: | , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology
2012
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3486612/ https://ncbi.nlm.nih.gov/pubmed/22869559 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.01182-12 |
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