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Detection and Differentiation of In Vitro-Spiked Bacteria by Real-Time PCR and Melting-Curve Analysis
We introduce a consensus real-time PCR protocol for the detection of bacterial DNA from laboratory-prepared specimens such as water, urine, and plasma. This prototype detection system enables an exact Gram stain classification and, in particular, screening for specific species of 17 intensive care u...
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Auteurs principaux: | , , , , , , |
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Format: | Artigo |
Langue: | Inglês |
Publié: |
American Society for Microbiology
2004
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Sujets: | |
Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC344435/ https://ncbi.nlm.nih.gov/pubmed/14766809 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.2.512-517.2004 |
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