Real-Time PCR for Detection and Differentiation of Gram-Positive and Gram-Negative Bacteria

We developed a consensus real-time PCR protocol that enables us to detect spiked bacterial 16S DNA from specimens such as water, urine, plasma, and sputum. The technique allows an exact Gram stain classification of 17 intensive care unit-relevant bacteria by means of fluorescence hybridization probe...

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Detalhes bibliográficos
Main Authors: Klaschik, Sven, Lehmann, Lutz E., Raadts, Ansgar, Book, Malte, Hoeft, Andreas, Stuber, Frank
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Bacteriology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC139680/
https://ncbi.nlm.nih.gov/pubmed/12409416
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.11.4304-4307.2002
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