Real-Time PCR for Detection and Differentiation of Gram-Positive and Gram-Negative Bacteria

We developed a consensus real-time PCR protocol that enables us to detect spiked bacterial 16S DNA from specimens such as water, urine, plasma, and sputum. The technique allows an exact Gram stain classification of 17 intensive care unit-relevant bacteria by means of fluorescence hybridization probe...

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Bibliografiske detaljer
Main Authors: Klaschik, Sven, Lehmann, Lutz E., Raadts, Ansgar, Book, Malte, Hoeft, Andreas, Stuber, Frank
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2002
Fag:
Bacteriology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC139680/
https://ncbi.nlm.nih.gov/pubmed/12409416
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.11.4304-4307.2002
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