Detection and Differentiation of In Vitro-Spiked Bacteria by Real-Time PCR and Melting-Curve Analysis

We introduce a consensus real-time PCR protocol for the detection of bacterial DNA from laboratory-prepared specimens such as water, urine, and plasma. This prototype detection system enables an exact Gram stain classification and, in particular, screening for specific species of 17 intensive care u...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Klaschik, S., Lehmann, L. E., Raadts, A., Book, M., Gebel, J., Hoeft, A., Stuber, F.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2004
Schlagworte:	Bacteriology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344435/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14766809/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.42.2.512-517.2004
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