Detection and Differentiation of In Vitro-Spiked Bacteria by Real-Time PCR and Melting-Curve Analysis

We introduce a consensus real-time PCR protocol for the detection of bacterial DNA from laboratory-prepared specimens such as water, urine, and plasma. This prototype detection system enables an exact Gram stain classification and, in particular, screening for specific species of 17 intensive care u...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Klaschik, S., Lehmann, L. E., Raadts, A., Book, M., Gebel, J., Hoeft, A., Stuber, F.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2004
Soggetti:	Bacteriology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC344435/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14766809/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.42.2.512-517.2004
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