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Species-Level Identification of Staphylococcal Isolates by Real-Time PCR and Melt Curve Analysis

A real-time PCR assay was developed to identify common staphylococcal species. A single set of consensus primers was designed to amplify a portion of the 16S rRNA gene, and a pair of fluorescence resonance energy transfer probes was used to identify species based on the unique melt properties of the...

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Detalhes bibliográficos
Main Authors: Skow, Áine, Mangold, Kathy A., Tajuddin, Mohammed, Huntington, Anne, Fritz, Brett, Thomson, Richard B., Kaul, Karen L.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1151902/
https://ncbi.nlm.nih.gov/pubmed/15956411
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.6.2876-2880.2005
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