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Detection of Carbapenemases by Real-Time PCR and Melt Curve Analysis on the BD Max System
We developed a multiplex SYBR green real-time PCR for the BD Max instrument (BD Diagnostics, Sparks, MD) to detect a panel of carbapenemases. The assay was evaluated with 152 consecutive isolates sent to the German National Reference Laboratory, and 65/65 of the carbapenemase-positive and 87/87 of t...
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| Autori principali: | , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology
2014
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3993711/ https://ncbi.nlm.nih.gov/pubmed/24523474 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00373-14 |
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