Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli

A real-time multiplex PCR assay was designed to amplify the virulence genes eae, pEAF, aatA, daaC, elt, est, ipaH, stx(1), and stx(2) for the detection of all diarrheagenic Escherichia coli pathotypes. This assay proved to be more sensitive and rapid than a conventional multiplex PCR for diarrheagen...

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Autors principals: Souza, Tamara B., Lozer, Diego M., Kitagawa, Sônia M. S., Spano, Liliana C., Silva, Neusa P., Scaletsky, Isabel C. A.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2013
Matèries:
Bacteriology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3592053/
https://ncbi.nlm.nih.gov/pubmed/23303494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02478-12
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