Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli

A real-time multiplex PCR assay was designed to amplify the virulence genes eae, pEAF, aatA, daaC, elt, est, ipaH, stx(1), and stx(2) for the detection of all diarrheagenic Escherichia coli pathotypes. This assay proved to be more sensitive and rapid than a conventional multiplex PCR for diarrheagen...

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Hlavní autoři: Souza, Tamara B., Lozer, Diego M., Kitagawa, Sônia M. S., Spano, Liliana C., Silva, Neusa P., Scaletsky, Isabel C. A.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2013
Témata:
Bacteriology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3592053/
https://ncbi.nlm.nih.gov/pubmed/23303494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02478-12
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