Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli

A real-time multiplex PCR assay was designed to amplify the virulence genes eae, pEAF, aatA, daaC, elt, est, ipaH, stx(1), and stx(2) for the detection of all diarrheagenic Escherichia coli pathotypes. This assay proved to be more sensitive and rapid than a conventional multiplex PCR for diarrheagen...

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Detalles Bibliográficos
Main Authors: Souza, Tamara B., Lozer, Diego M., Kitagawa, Sônia M. S., Spano, Liliana C., Silva, Neusa P., Scaletsky, Isabel C. A.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2013
Assuntos:
Bacteriology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3592053/
https://ncbi.nlm.nih.gov/pubmed/23303494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02478-12
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