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Rapid Detection and Identification of Metallo-β-Lactamase-Encoding Genes by Multiplex Real-Time PCR Assay and Melt Curve Analysis

Metallo-β-lactamase enzymes (MβL) are encoded by transferable genes, which appear to spread rapidly among gram-negative bacteria. The objective of this study was to develop a multiplex real-time PCR assay followed by a melt curve step for rapid detection and identification of genes encoding MβL-type...

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Detalhes bibliográficos
Main Authors: Mendes, Rodrigo E., Kiyota, Katia A., Monteiro, Jussimara, Castanheira, Mariana, Andrade, Soraya S., Gales, Ana C., Pignatari, Antonio C. C., Tufik, Sergio
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1829038/
https://ncbi.nlm.nih.gov/pubmed/17093019
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01728-06
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