Detection and Differentiation of In Vitro-Spiked Bacteria by Real-Time PCR and Melting-Curve Analysis

We introduce a consensus real-time PCR protocol for the detection of bacterial DNA from laboratory-prepared specimens such as water, urine, and plasma. This prototype detection system enables an exact Gram stain classification and, in particular, screening for specific species of 17 intensive care u...

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Bibliographic Details
Main Authors: Klaschik, S., Lehmann, L. E., Raadts, A., Book, M., Gebel, J., Hoeft, A., Stuber, F.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2004
Subjects:
Bacteriology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC344435/
https://ncbi.nlm.nih.gov/pubmed/14766809
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.2.512-517.2004
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