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Structural analyses of covalent enzyme-substrate analogue complexes reveal strengths and limitations of de novo enzyme design

We report the cocrystal structures of a computationally designed and experimentally optimized retro-aldol enzyme with covalently bound substrate analogs. The structure with covalently bound substrate analog is similar but not identical to the design model, with an RMSD over the active site residues...

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Détails bibliographiques
Auteurs principaux: Wang, Ling, Althoff, Eric A., Bolduc, Jill, Jiang, Lin, Moody, James, Lassila, Jonathan K., Giger, Lars, Hilvert, Donald, Stoddard, Barry, Baker, David
Format: Artigo
Langue:Inglês
Publié: 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3440004/
https://ncbi.nlm.nih.gov/pubmed/22075445
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2011.10.043
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