Structural analyses of covalent enzyme-substrate analogue complexes reveal strengths and limitations of de novo enzyme design

We report the cocrystal structures of a computationally designed and experimentally optimized retro-aldol enzyme with covalently bound substrate analogs. The structure with covalently bound substrate analog is similar but not identical to the design model, with an RMSD over the active site residues...

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Hlavní autoři: Wang, Ling, Althoff, Eric A., Bolduc, Jill, Jiang, Lin, Moody, James, Lassila, Jonathan K., Giger, Lars, Hilvert, Donald, Stoddard, Barry, Baker, David
Médium: Artigo
Jazyk:Inglês
Vydáno: 2011
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3440004/
https://ncbi.nlm.nih.gov/pubmed/22075445
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2011.10.043
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