Overproduction of the EcoR V endonuclease and methylase.

Strains overproducing the EcoR V endonuclease and methylase have been obtained by inserting each of the two genes in expression vectors containing the lambda PL promoter. The methylase is overproduced to a level reaching 5-10% of the total cellular proteins, which represents a 50-100 fold increase....

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Bougueleret, L, Tenchini, M L, Botterman, J, Zabeau, M
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1985
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341280/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2989776/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.11.3823
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