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Measuring motion on DNA by the type I restriction endonuclease EcoR124I using triplex displacement

The type I restriction enzyme EcoR124I cleaves DNA following extensive linear translocation dependent upon ATP hydrolysis. Using protein-directed displacement of a DNA triplex, we have determined the kinetics of one-dimensional motion without the necessity of measuring DNA or ATP hydrolysis. The tri...

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Bibliografski detalji
Izdano u:EMBO J
Glavni autori: Firman, Keith, Szczelkun, Mark D.
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group 2000
Teme:
Online pristup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC305691/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10790375/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/19.9.2094
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