Measuring motion on DNA by the type I restriction endonuclease EcoR124I using triplex displacement

The type I restriction enzyme EcoR124I cleaves DNA following extensive linear translocation dependent upon ATP hydrolysis. Using protein-directed displacement of a DNA triplex, we have determined the kinetics of one-dimensional motion without the necessity of measuring DNA or ATP hydrolysis. The tri...

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Veröffentlicht in:	EMBO J
Hauptverfasser:	Firman, Keith, Szczelkun, Mark D.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Nature Publishing Group 2000
Schlagworte:	Articles
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC305691/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10790375/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/19.9.2094
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