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Measuring motion on DNA by the type I restriction endonuclease EcoR124I using triplex displacement
The type I restriction enzyme EcoR124I cleaves DNA following extensive linear translocation dependent upon ATP hydrolysis. Using protein-directed displacement of a DNA triplex, we have determined the kinetics of one-dimensional motion without the necessity of measuring DNA or ATP hydrolysis. The tri...
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| Main Authors: | , |
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| פורמט: | Artigo |
| שפה: | Inglês |
| יצא לאור: |
Oxford University Press
2000
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| נושאים: | |
| גישה מקוונת: | https://ncbi.nlm.nih.gov/pmc/articles/PMC305691/ https://ncbi.nlm.nih.gov/pubmed/10790375 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/19.9.2094 |
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