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Overproduction of the EcoR V endonuclease and methylase.

Strains overproducing the EcoR V endonuclease and methylase have been obtained by inserting each of the two genes in expression vectors containing the lambda PL promoter. The methylase is overproduced to a level reaching 5-10% of the total cellular proteins, which represents a 50-100 fold increase....

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Auteurs principaux: Bougueleret, L, Tenchini, M L, Botterman, J, Zabeau, M
Format: Artigo
Langue:Inglês
Publié: 1985
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC341280/
https://ncbi.nlm.nih.gov/pubmed/2989776
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