Overproduction of the EcoR V endonuclease and methylase.
Strains overproducing the EcoR V endonuclease and methylase have been obtained by inserting each of the two genes in expression vectors containing the lambda PL promoter. The methylase is overproduced to a level reaching 5-10% of the total cellular proteins, which represents a 50-100 fold increase....
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| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
1985
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| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341280/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2989776/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.11.3823 |
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