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A deletion mutant of the type IC restriction endonuclease EcoR1241 expressing a novel DNA specificity.

We have developed a complementation assay which allows us to distinguish between mutations affecting subunit assembly and mutations affecting DNA binding in the DNA recognition subunit (HsdS) of the multimeric restriction endonuclease EcoR1241. A number of random point mutations were constructed to...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Abadjieva, A, Patel, J, Webb, M, Zinkevich, V, Firman, K
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1993
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC311173/
https://ncbi.nlm.nih.gov/pubmed/8233776
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