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Overproduction of the EcoR V endonuclease and methylase.

Strains overproducing the EcoR V endonuclease and methylase have been obtained by inserting each of the two genes in expression vectors containing the lambda PL promoter. The methylase is overproduced to a level reaching 5-10% of the total cellular proteins, which represents a 50-100 fold increase....

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:Nucleic Acids Res
Hauptverfasser: Bougueleret, L, Tenchini, M L, Botterman, J, Zabeau, M
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 1985
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC341280/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2989776/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.11.3823
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